Search results for "in vial automated derivatization"

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External Influences on Invertebrate Brain Histamine and Related Compounds via an Automated Derivatization Method for Capillary Electrophoresis.

2017

8 pages; International audience; Histamine has been shown to modulate visual system and photic behavior in arthropods. However, few methods are available for the direct quantification of histamine and its precursor and metabolites in arthropod brain. In this work, a method for the separation of histamine, its precursor histidine, and its metabolite N-methyl-histamine from brain extracts of a freshwater crustacean has been developed using capillary electrophoresis with laser-induced fluorescence detection. Molecules were tagged on their primary amine function with naphthalene-2,3-dicarboxaldehyde, but derivatized histamine and N-methyl-histamine exhibited poor stability in contrast to deriva…

0301 basic medicinePhysiologyCognitive NeuroscienceMetabolitelaser-induced fluorescence detectioninvertebratecapillary electrophoresisBiochemistry[ SDV.EE ] Life Sciences [q-bio]/Ecology environment03 medical and health scienceschemistry.chemical_compound0302 clinical medicineCapillary electrophoresisRiversin vial automated derivatizationAnimalsAmphipodaHistidineDerivatizationHistidine[SDV.EE]Life Sciences [q-bio]/Ecology environmentDetection limitAutomation LaboratoryChromatographyMethylhistaminesBrainElectrophoresis CapillaryReproducibility of ResultsCell BiologyGeneral MedicinecrustaceaFluorescence030104 developmental biologychemistry[ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]Calibration[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]Amine gas treatingSeasons030217 neurology & neurosurgeryHistamineHistamineACS chemical neuroscience
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